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biotinylated anti human mgl antibody  (R&D Systems)


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    R&D Systems biotinylated anti human mgl antibody
    Biotinylated Anti Human Mgl Antibody, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/biotinylated+anti+human+mgl+antibody/Human+CLEC10A%2FCD301+Biotinylated+Antibody/pm41006428-75-14-21
    Average 93 stars, based on 1 article reviews
    biotinylated anti human mgl antibody - by Bioz Stars, 2026-09
    93/100 stars

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    Incubation:

    Article Title: Amniotic fluid glycoproteins as potential ligands for macrophage galactose-type C-type lectin and their possible implications for immunoregulation during pregnancy
    Article Snippet: The membranes were blocked overnight using 15 mM Tris–HCl buffer, pH 7.4, with 0.15 M NaCl (TBS) with 1% Tween-20 solution and then incubated with: (1) recombinant human MGL (CLEC10a) protein (Elabscience, Houston, Texas, USA) diluted 1:500 in 15 mM Tris–HCl buffer, pH 7.4, with 0.15 M NaCl; 0.1% Tween-20 (TBST) containing 2 mM Ca 2+ or additionally 25 mM GalNAc; (2) biotinylated lectin from Vicia villosa (VVL, Vector Labs, Burlingame, CA, USA) diluted 1:200 in TBST; and (3) biotinylated lectin from Wisteria floribunda (WFL, Vector Labs, Burlingame, CA, USA) diluted 1:500 in TBST, for 1 h at room temperature (RT) with constant gentle shaking. .. To detect glycoprotein-MGL complexes, the membranes were incubated for 1 h at RT with biotinylated anti-human MGL antibody (anti-human CLEC10A/CD301; BAF4888, R&D Systems, Minneapolis, MN, USA), diluted 1:2000 in TBST. .. For all lectins (MGL, VVL, and WFL), the complexes were detected with ExtrAvidin-alkaline phosphatase (Sigma-Aldrich, St. Louis, MO, USA), diluted 1:10,000 in TBST, and incubated for 45 min at RT, followed by development with BCIP/NBT substrate (Sigma-Aldrich, St. Louis, MO, USA).

    Article Title: Amniotic fluid glycoproteins as potential ligands for macrophage galactose-type C-type lectin and their possible implications for immunoregulation during pregnancy.
    Article Snippet: The membranes were blocked overnight using 15 mM Tris–HCl buffer, pH 7.4, with 0.15 M NaCl (TBS) with 1% Tween-20 solution and then incubated with: (1) recombinant human MGL (CLEC10a) protein (Elabscience, Houston, Texas, USA) diluted 1:500 in 15 mM Tris–HCl buffer, pH 7.4, with 0.15 M NaCl; 0.1% Tween-20 (TBST) containing 2 mM Ca2+ or additionally 25 mM GalNAc; (2) biotinylated lectin from Vicia villosa (VVL, Vector Labs, Burlingame, CA, USA) diluted 1:200 in TBST; and (3) biotinylated lectin from Wisteria floribunda (WFL, Vector Labs, Burlingame, CA, USA) diluted 1:500 in TBST, for 1 h at room temperature (RT) with constant gentle shaking. .. To detect glycoprotein-MGL complexes, the membranes were incubated for 1 h at RT with biotinylated anti-human MGL antibody (anti-human CLEC10A/CD301; BAF4888, R&D Systems, Minneapolis, MN, USA), diluted 1:2000 in TBST. .. For all lectins (MGL, VVL, and WFL), the complexes were detected with ExtrAvidin-alkaline phosphatase (Sigma-Aldrich, St. Louis, MO, USA), diluted 1:10,000 in TBST, and incubated for 45 min at RT, followed by development with BCIP/NBT substrate (SigmaAldrich, St. Louis, MO, USA).



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    93
    R&D Systems biotinylated anti human mgl antibody
    Biotinylated Anti Human Mgl Antibody, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/biotinylated+anti+human+mgl+antibody/Human+CLEC10A%2FCD301+Biotinylated+Antibody/pm41006428-75-14-21
    Average 93 stars, based on 1 article reviews
    biotinylated anti human mgl antibody - by Bioz Stars, 2026-09
    93/100 stars
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